Journal: bioRxiv

Article Title: Direct Binding of miR-155 to FLT3 Regulates Key Cellular Functions in Acute Myeloid Leukemia

doi: 10.64898/2026.01.15.696285

Figure Lengend Snippet: A. miR-155 expression levels were quantified in MV4-11, Molm-13, THP-1 cell lines by qPCR, and U6 expression was used as endogenous control. B. mRNA expression levels of FLT3 in MV4-11, Molm-13, THP-1 cell lines. The GAPDH gene was used as an endogenous control. C. Surface and D. intracellular expression of CD135 (FLT3) in MV4-11 and THP-1 cells. Cells were stained with anti-human CD135-PE, and unstained control was used to rule out any background noise posed by cell autofluorescence. Data are presented as Mean ±SEM of 3 independent experiments, and qPCR data represented as fold-change.

Article Snippet: MV4-11 cells were seeded in 6-well plate until seeding density reached 60-80% and transfected with 50 nM human miR-155-5p inhibitor (hereafter referred to as AntimiR-155) and AntimiR-155 negative inhibitor control (hereafter referred to Anti-miR155 N.Ctrl) oligonucleotides using RNAimax lipofectamine transection reagent (Thermo Fisher Scientific, Inc.), following the manufacturer’s instructions, and incubated at 37°C with 5% CO 2 for 24h.

Techniques: Expressing, Control, Staining

Journal: bioRxiv

Article Title: Direct Binding of miR-155 to FLT3 Regulates Key Cellular Functions in Acute Myeloid Leukemia

doi: 10.64898/2026.01.15.696285

Figure Lengend Snippet: A. Bioinformatics analysis predicted the presence of a putative binding site in FLT3 mRNA 3’-UTR. In silico sequence complementary pairwise analysis with 7-mer match between miR-155 seeding region and FLT3 mRNA. B. Multiple sequence alignment of miR-155 seeding region across different species. C. Cell viability detected using trypan blue exclusion assay after 24h of miR-155 knockdown in MV4-11 cells using 50 nM antimR-155 or antimiR-155 control (Ctrl). D. miR-155 expression levels by qPCR in MV4-11 cells 24h post-treatment with 50 nM antimiR-155 or antimiR-155 Ctrl. E. FLT3 mRNA expression levels in MV4-11 cells 24h post-treatment with 50 nM antimiR-155 or antimiR-155 Ctrl. The GAPDH and U6 genes were used as endogenous controls in FLT3 and miR-155 qPCR respectively, and qPCR data represented as fold-change. F. FLT3 protein expression 24h post-treatment with 50 nM antimiR-155 (red) or antimiR-155 Ctrl (blue). Live cells were stained with anti-human CD135-PE (FLT3) and analyzed using flowcytometry.

Article Snippet: MV4-11 cells were seeded in 6-well plate until seeding density reached 60-80% and transfected with 50 nM human miR-155-5p inhibitor (hereafter referred to as AntimiR-155) and AntimiR-155 negative inhibitor control (hereafter referred to Anti-miR155 N.Ctrl) oligonucleotides using RNAimax lipofectamine transection reagent (Thermo Fisher Scientific, Inc.), following the manufacturer’s instructions, and incubated at 37°C with 5% CO 2 for 24h.

Techniques: Binding Assay, In Silico, Sequencing, Trypan Blue Exclusion Assay, Knockdown, Control, Expressing, Staining

Journal: bioRxiv

Article Title: Direct Binding of miR-155 to FLT3 Regulates Key Cellular Functions in Acute Myeloid Leukemia

doi: 10.64898/2026.01.15.696285

Figure Lengend Snippet: A. MV4-11 cell proliferation 24h post-treatment with 50 nM antimiR-155 or antimiR-155 Ctrl. Cells were treated with indicated dose of antimiR-155 or Ctrl and seeded in 96-well plate and incubated 24h. Cell proliferation was then measured using CCK-8 assay. B. Colony formation assay was performed after cells were treated with 50 nM antimiR-155 or antimiR-155 Ctrl and seeded in a ratio of 1:10 cells to methylcellulose media and then incubated for 7 days. Visible colonies were counted macroscopically and expressed as a number of colonies produced by the cells. C. MV4-11 cell apoptosis was measured by flow cytometry utilizing annexin V and PI markers 24h post-treatment with 50 nM antimiR-155 or antimiR-155 Ctrl. Data represent the Mean ±SEM; significant differences relative to control group are shown as *p<0.05 of three independent experiments.

Article Snippet: MV4-11 cells were seeded in 6-well plate until seeding density reached 60-80% and transfected with 50 nM human miR-155-5p inhibitor (hereafter referred to as AntimiR-155) and AntimiR-155 negative inhibitor control (hereafter referred to Anti-miR155 N.Ctrl) oligonucleotides using RNAimax lipofectamine transection reagent (Thermo Fisher Scientific, Inc.), following the manufacturer’s instructions, and incubated at 37°C with 5% CO 2 for 24h.

Techniques: Incubation, CCK-8 Assay, Colony Assay, Produced, Flow Cytometry, Control